Urinary Tract Infection (UTI) are among the most common bacterial infections that lead patients to seek medical care. Nitrofurantoin is an excellent option for treatment of acute uncomplicated UTI. Aim of the study is to determine the sensitivity profile of Nitrofurantoin in patients with UTI and compare it with sensitivity profile of Norfloxacin and Cotrimoxazole. Overall sensitivity to Nitrofurantoin Norfloxacin and Cotrimoxazole in this study was found to be 67% 27% and 47%. Sensitivity of Escherichia coli to Nitrofurantoin was 84%. Nitrofurantoin is an excellent option for treating acute uncomplicated UTI on OPD basis.