Background: The risk of acquiring Hepatitis B the infection is more in health care worker than in general population. The nurses are exposed to this infection due to their work. Methodology: An observational cross sectional study was carried out among nursing staff of R G Kar Medical College with the help of pre designed semi structured questionnaire. Data on demographic characteristics, knowledge, occupational exposure, vaccination status were collected. Result: Among study population 57.3% were completely vaccinated & 12.5% incompletely vaccinated. Satisfactory knowledge was found in 68.9% of the nursing staff. The major reason for incomplete and non vaccination was non availability through government sources and forgetfulness. Conclusion: Hepatitis B vaccination coverage was not satifactory. Mandatory vaccination at the time of their training & frequent educational program about the disease is very much required.