Aims: To evaluate antinociceptive activity of ethanolic neem leaf extract (ENLE) and determine involvement of opioidergic pathway in its effect. Methods and Material: The antinociceptive activity of ENLE was studied using hot plate method. Two different doses of ENLE (50mg/kg and 100 mg/kg, p.o.) were administered in adult rats. Morphine (1 mg/kg, s.c.) was used as reference drug. To determine opioidergic pathway, pre–treated rats with naloxone (1mg/kg, i.p.) were used. Statistical analysis used: Statistical analysis was done by one way ANOVA followed by Least significant difference test. Results: ENLE shows antinociceptive effect in dose dependent manner as compared to control (p<0.01). When compared with morphine, morphine shows significant difference in its activity (p<0.05 and p<0.01) as compared with ENLE. Effects were reduced in naloxone pre–treated rats. Conclusion: ENLE exhibited antinociceptive activity through central mechanism as compared with control. Involvement of opioidergic pathway is suggested.