Aim and Objective: The aim of this study was to establish a simple criterion for improving the pregnancy rate by assessing the early cleavage in Intra Cytoplasmic Sperm Injection (ICSI). Materials and Methods: A total of 294 patients enrolled for ICSI fulfilling the selection criteria were recruited for the study at a tertiary care assisted reproductive centre. ICSI was performed 2–3 h after oocyte aspiration with the prepared sperm. All embryos were checked for early cleavage at 27 hours post ICSI. They were divided into two groups. Group I embryos which cleaved before 27 hours after ICSI. Group II– embryos which cleaved after 27 hours. The pregnancy rates were compared between the two groups. Results: All the 294 patients were analysed. There was no difference in the mean age, duration of ovarian stimulation, number of oocytes retrieved, fertilization, cleavage rates and embryo quality between the two groups. Early cleavage was observed in 165 patients (56.12 %). Late cleavage was observed in 129 patients (43.88%). The pregnancy was confirmed in 129 patients out of 165 (78.20 %) in Group I and 50 patients out of 129 (38.80%) in Group II which was statistically significant P <0.005. Conclusion: Simple selection criteria using the early cleavage embryo may improve the pregnancy rate in ICSI.