Background: The contribution of staining techniques in brightfield microscopy has been remarkable, considering that many of these staining techniques are still widely used for diagnostic purposes more than a century after their introduction. Each working day in laboratories around the world, millions of microscope slides stained with Hematoxylin and Eosin are prepared and viewed by pathologists as part of the diagnostic process. Materials and Methods: The study included histological sections of four groups and 3 hematoxylin solutions. Each group of hematoxylin had sections of four groups. Hence, a total number of 60 sections were made. Results: The analysis of the relationship of the following variables, namely, different alum hematoxylin concluded that Harri’s hematoxylin was superior to Mayer’s and Ehrlich hematoxylin. Conclusion: Our study has shown much promise in exploring Hematoxylin and Eosin stain as a routine staining procedure. The persistence and continuing viability and growth of Hematoxylin and Eosin morphology indicates that this simple technique continues to meet most of the requirements of not only the pathologists but also clinicians, and, let us not forget, patients.