Objective As Neonatal sepsis is a major cause of morbidity and mortality of newborns (< 1 month of age) we have done this study to study the sociodemographic and microbiological profile of neonatal sepsis and antibiotic susceptibility pattern of the isolates from neonates admitted in a tertiary hospital in central India. Results We studied a total of 200 suspected cases of neonatal sepsis, out of which 30 (15%) cases showed positive blood culture. The prevalent of positive blood culture with different neonatal risk factors (sex, age, birth weight, gestational age, and delivery mode) showed highest positive bacterial growth in male (60%); 3 or above 3 days age (70%); low birth weight (67%); preterm gestational age (37%); and caesarean delivery mode (63%). Among positive cases, the bacteriological profile was found highest for Staphylococcus aureus (40%) followed by Klebsiella pneumoniae (16%).