Aim: To evaluate effect of different temperatures on the physical properties like flow and setting time of resin based sealer, silicone based sealer and MTA based sealer . Materials and methods: Three different root canal sealers i.e. Resin based, Silicone based, MTA based were tested to evaluate the flow and final setting time. 10 samples of each of the sealers were packed in stainless steel ring moulds, after exposing to different temperatures, setting time of these sealers were determined using Vicats needle apparatus. To test the flow of sealers 0.05 ml of the sealer was dispensed on to the glass slab, another glass slab along with weights were placed over it. The minor and major diameter of sealer were measured using digital calliper. Paired t–test and One way Anova test were used to analyse the data. Results: Silicone based sealers had highest flow at normal temperatures whereas when the temperatures were raised to 140℃ silicone based sealers showed least flow. Setting time at 37 °C for all 3 sealers remained unchanged. On increasing temperatures at 140°C, the setting time of Silicone based sealer was reduced compared to that of Resin based sealers while MTA based sealers remained unaffected. Conclusion: In this study Resin based sealers and Silicone sealer showed changes in the physical properties (setting time and flow) at high temperatures. Flow of the sealers were also influenced at different temperatures whereas MTA based remained unaffected at all temperatures.