Background : Influenza A H1N1is an infectious disease caused by RNA viruses of the family Orthomyxoviridae (the influenza viruses). Methods: Retrospective study was conducted on all the suspected cases of Influenza A H1N1 (Swine Flu) from October 2018 to September 2019 in the Department of Microbiology, Government Medical College, Kota, Rajasthan, India. Results: Out of 3979 suspected cases, 606 were tested positive among which 51.32% were males and 48.68% were females. Maximum prevalence was noted among age group 0–10 years (18.67%) followed by 41–50 years of age group (18.26%).. There might exists a relation between the humidity and the virus activity. Interpretation and Conclusion: Conscientious screening and expeditious management and other preventive measures for identifying the suspected cases and isolating them will go a long way in curtailing the recurrence of this epidemic