Objective: Nanoparticles synthesized using variety of hazardous chemical methods which are not environmentally friendly. Hence, the present study was designed to synthesize silver nanoparticles biologically using peel extract of Citrus reticulata and characterization of biosynthesized silver nanoparticle. Methods: In our study silver nanoparticles were synthesized with peel extract of Citrus reticulata fruit and aqueous solution of 1 mM silver nitrate solution and then characterized using UV–Visble, Fourier transform infrared spectroscopy (FTIR), X–ray diffraction (XRD), and Scanning electron microscopic (SEM) methods. Absorption maxima for silver colloidal solution showed at 420 nm in a UV–visible spectrum. The functional biomolecules such as carboxyl groups responsible for the silver nanoparticles formation were characterized by FTIR. Results: The x–ray diffraction results revealed that the crystallization of the bioorganic phase occurs on the surface of the silver nanoparticles or vice–versa. The broadening of peaks in the x–ray diffraction patterns was ascribed to particle size effects. In our study, presence of elemental silver was proven by EDX analysis and x–ray diffration evidenced that the silver ions have been reduced to elemental silver. In conclusion, our study demonstrated simple benign, cost–effective biosynthesis methods of silver nanoparticles using peel extract of Citrus reticulata fruits. Conclusion: our findings could be explored for industrial production of nanoparticles and their use in biomedical and pharmacy applications