Hand hygiene is a milestone of infectious disease control and its promotion has been recognized as an important public health measure. Due to constant relationship with patients, nurses play a paramount role in proper execution of hand hygiene. A study was conducted to assess the knowledge of nursing students and their performance was measured before and after the workshop. The knowledge score regarding hand hygiene was significantly improved from 4.34±1.5 to 8.84±0.9. Emphasis on infection control to lower hospital acquired infections (HAI) should be considered as an important aspect and must be included as educational priority.