Glucose –6–phosphate dehydrogenase (G6PD) is the rate limiting enzyme of pentose phosphate pathway which produce NADPH , involved in lipid biosynthesis. Insulin is an anabolic hormone having profound influence on various key enzymes involved in lipid metabolism. The current study was designed to evaluate serum G6PD activity and its association with dyslipidemia in Type 2 DM. Total 100 diabetic patients and 100 age and sex matched healthy controls were included in the study. Blood G6PD was measured by chemical method. Estimation of cholesterol , TG , HDL, LDL was done in autoanalyzer . Statistical analysis was done by SPSS Version 20 software. Mean G6PD levels was significantly higher in diabetic cases . Increased G6PD levels correlates positively with TC,TG,LDL, however HDL shows a negative correlation. Increased activity of G6PD in diabetic cases, alters lipid homeostasis which leads to atherogenic abnormalities. Estimation of serum G6PD & its correlation with dyslipidemia can highlight its role in regulating lipid status in diabetes mellitus patients .