Introduction: Haemoglobinopathies are a group of disorders of hemoglobin. Inherited abnormalities of Hemoglobin synthesis may be divided into two groups: (1) Structurally abnormal hemoglobin variants. (2) One or more of the normal hemoglobin are synthesized at reduced rate. Aims and Objectives: 1. To assess the pattern of Thalassemia syndrome and Other Haemoglobinopathies by HPLC (High Performance Liquid Chromatography) method in patients of Civil Hospital, Ahmedabad. 2. To study age and gender wise distribution of Haemoglobinopathies in patients of Civil Hospital, Ahmedabad. 3. To study the incidence of Haemoglobinopathies in different castes in patients of Civil Hospital, Ahmedabad according to the caste. 4. To compare the results of present study with the results of other authors similar studies. Material and Method: The study has been carried out as “Time bound study” to find the pattern of β–Thalassemia and Other Hemoglobinopathies for a period of two years from October 2015 to November 2017. Observation and Result: Most common HPLC findings in this study was Beta thalassemia Minor (38.47%) followed by Sickle cell trait (21.43%), Sickle cell disease (17.14%), Beta Thalassemia Major (5.71%), Sickle–β thalassaemia (5.71%) and Beta Thal. Intermedia (4.29%). Conclusion: HPLC is easy, reproducible & accurate in most cases of haemoglobinopathies.