Aim: To isolate and identify the fungal isolates from various clinically diagnosed cases of pulmonary infections with history of diabetes mellitus. Material and Methods: Sputum samples from various clinically diagnosed cases of pulmonary infections with history of diabetes mellitus were studied for fungal isolates. Samples were tested in the laboratory for fungus by potassium hydroxide preparation (KOH preparation) and fungal culture. Results: Out of the total of 100 sputum samples , sixty two samples (62%) were positive for fungus. Out of these positive samples, Aspergillus species (50%) was found to be the commonest isolate followed by Candida species (38.7%), Rhizopus species (9.7%) and Mucor species (1.6%). Conclusion: Following Aspergillus species and Candida species, the third leading fungal isolate in various pulmonary infections is Rhizopus species, especially in diabetic patients. Mycological examination aids in the diagnosis of various pulmonary infections for their effective management