Background: Staphylococcus aureus is a major human pathogen that causes wide range of clinical infections. Methicillin–resistant Staphylococcus aureus(MRSA) is endemic in India and is a dangerous pathogen causing hospital acquired infection leadings to significant morbidity and mortality. Objective: To study the profile of Staphylococcus aureus isolated from patients admitted in a tertiary care hospital. Result: Majority of clinical isolates of S.aurueswas obtained from patients of skin and soft tissue infection(54.66%) followed by those suffering from respiratory infection (13.33%), blood stream infection (13.33%) and UTI(8%). S.aureus was predominantly isolated from IPD samples, maximum cases were in the age group of 31–40 years and males outnumbered females. There was predominance of MRSA 112 (74.66%)which showed high level of resistance to penicillin (100%), ciprofloxacin (82.14 %), co–trimoxazole (79.46%) and moxifloxacin(85.71%). All the clinical isolates of S.aureuswere sensitive to linezolid andvancomycin (MIC <1ugm/ml).Conclusions: The clinical isolates of S.aureusshowed high level of resistance to various antimicrobial agents which is a significant nosocomial threat. Surveillance and infection control practices should be carried out to prevent cross transmission of such resistant pathogen within the hospital setting.