Extended–spectrum β–lactamases (ESBL) producing micro–organism increases infection in catheter associated (CAUTI) urinanary tract infection and led to limitations of treatment options. The aim of our study is to know the prevalence of ESBL producing E. coli spp. at a tertiary hospital in north bihar. Methods: A total of 250 mid–stream urine (MSU) samples were collected during the study period from October 2017 to September 2019. Identification of the isolates was done by Gram‘s staining followed by biochemical tests. ESBL screening among E. coli spp. isolates were done by phenotypic disc diffusion test method (combined disc method) using ceftazidime (30µg) and ceftazidime plus clavulanic acid (30/10µg). Results: A total of 150 samples showed significant bacteriuria with 30 (20 %) E. coli. By combined disk test, 20 (66.6%) E. coli spp. were found ESBL producers. Conclusion: Prevalence of ESBL producing E. coli spp. is high among catheter associated urinary tract infection. Prompt effective diagonosis and management of MDR strains is necessary in clinical setting to decrease morbidity among admitted patients .