Spontaneous bacterial peritonitis(SBP) is infection of ascitic fluid particularly common in chronic liver disease. Patients with chronic liver disease with compensated cirrhosis develops ascites. Due to inadequate defense mechanism chronic liver disease patients with ascites are susceptible to infection. Our study done to know the microbiological profile of spontaneous bacterial peritonitis in chronic liver disease patients. This was done over a period of two year from October 2017 to September 2019. Ascitic fluid sample collected aseptically, culture on blood agar and Mac Conkey agar. Positive cases obtained on culture were included in this study. Isolates are identified by standard protocol as per standard bacteriological technique. Out of 150, 60 diaognosed as culture positive cases in spontaneous bacterial peritonitis patients. Bacterial isolates recovered from ascitic fluid sample, most commonly isolated species are E.coli, Klebsiella spp. , Staphylococcus aureus ,Pseudomonas spp. and Acinetobacter baumanii . In our hospital chronic liver disease is most common cause of ascites and Culture positive E. Coli is most common isolate in spontaneous bacterial peritonitis (SBP) pts