The sexually transmitted diseases are transmitted predominantly by sexual contact. Genital herpes simplex virus infection is the second most prevalent STI worldwide. The present study was carried out in a Tertiary care hospital of Central India, from Oct 2015 to Sept 2017. The study group consisted of 102 syndromically diagnosed cases of Genital Ulcers who visited the SURAKSHA CLINIC Out of 102 cases, 67.65% were of Herpetic Ulcers , 32.35% were of Non Herpetic Ulcers. Out of 102 serum samples, 76.47% samples were positive for IgG antibody and 20.59% were positive for IgM antibody, 11.76% samples were positive for both antibodies. Out of 87 seropositive cases for HSV–2, 10.53% cases were coinfected with HIV. HSV–2 infections are common and have spread worldwide. The present study was done to determine the seroprevalence of HSV–2 among GUD’s and its association with other demographic and behavioural factors