Background: Otomycosis is fungal infection of the external auditory canal and its associated complications sometimes involving the middle ear. It occurs because the protective lipid/acid balance of the ear is lost. Materials and methods: The present retrospective observational study was carried out in the Department of ENT & Head and Neck Surgery, S.M.G.S Hospital, Govt. Medical College, Jammu for a period of one year w.e.f. January 2019 to January 2020. The study included 100 patients of all age groups and both the sexes presenting with symptoms like itching, pain, feeling of blocked ear, tinnitus, deafness, and otoscopy revealing masses of hyphae/spores or a curd–like grey/white discharge were included. Age, sex, socioeconomic status, and occupation of the patient were also recorded. The material from the deeper portion of the EAC was taken using sterile aural swabs and sent to microbiology department for processing. One swab was subjected to microscopic examination with10% KOH and the other swab was inoculated over Sabouraud’s dextrose agar media for culture. All patients diagnosed with otomycosis were subjected to a thorough aural toilet by suctioning and removal of the fungal debris. Results: A total of 100 patients were studied. Maximum patients(29%)were in the age group of 31– 40 years.64(64%) patients were males and 36(36%) patients were females with male: female ratio of 1.7: 1.The most common presenting symptom was itching in the ears seen in 78(78%) patients, followed by sensation of ear blockage(64%), ear discharge(59%), hearing impairment(55%), pain(30%) and ringing in ears(28%). Otomycosis was predominantly unilateral both in males and in females. Only 10(10%)patients presented with bilateral infection.The most common fungal isolates belonged to the species of Aspergillus accounting for 87% of all fungal isolates. Conclusion: Aspergillus spp. were the most common fungi isolated.