Background: The liver plays the dominant part in thyroid hormone metabolism. Normal circulating thyroid hormone levels are essential for both normal hepatic circulation and normal bilirubin metabolism. Study aimed to estimate the liver enzymes AST, ALT and ALP in overt and subclinical hypothyroidism and to compare it to that of apparently normal euthyroid controls. Material and method: Institutional ethics clearance was obtained for this present cross sectional observational study which was conducted on 50 patients of hypothyroidism attending OPD clinics at Bowring and Victoria Hospitals between the age of 18–55 years. Among them 20 were diagnosed with overt hypothyroidism and 30 with subclinical hypothyroidism. To compare, a 50 Healthy subjects were included as controls. Venous blood samples collected in plain clot activated tube from cases and controls after obtaining the informed consent under all aseptic precautions. The sample was centrifuged after 15 mins and was analyzed for serum levels of T3, T4, TSH, AST, ALT and ALP on fully auto–analyser. Result: Study included a total of 100 patients, among whom 50 were healthy controls and other 50 were with hypothyroidism. The mean age of hypothyroid subjects was 45.31 ± 4.12 years and that of euthyroid normal subjects was 44.95 ± 4.55 years. Hypothyroid consisted of overall 75% women were as the euthyroid group had 72% women. Mean AST levels in cases (35.62 ± 5.31 IU/L) was significantly higher (P < 0.01) as compared to controls (24.06 ± 4.40 IU/L) Conclusion: Hypothyroidism has association with increase in AST levels and ALT, ALP levels and such patients should be monitored regularly for liver function. Early detection and treatment will prevent further disorder–related complications and will be beneficial during thyroid patient care