Background: Neonates receive only small amounts of fluids in the first days following birth, and they tend to lose weight before they begin to gain weight. The Indian data on neonatal weight loss and prevalence of AKI is scarce. Thus, the present study was planned to assess weight loss in early neonatal life, and evaluation of AKI and hypernatremia in such patients. Materials and Methods: The present study was of cross sectional design, which was carried out a tertiary care hospital, Hingna, Nagpur. Babies beyond 34 weeks of gestation with weight loss of >8% during early neonatal period with exclusive breast feeding were included in the study. Results: 151 patients fulfilled the inclusion criteria and were taken in the study. On analyzing the age and sex–wise distribution of neonates, it was found that majority of them had gestational age of >37 weeks comprising of 91 neonates (60%), while 59 (40%) belonged to gestation age 34–37 weeks. On analyzing the weight loss and age of the neonate, it was found that weight loss was more in neonates aged 3–7 days as compared to those <3 days across all categories of birth weight. Stage 1 AKI was seen in all neonates with weight loos of 8–10%, while in neonates with weight loss of 10–15%, 16 (92%) had stage 1 AKI. Neonates with weight loss >15 showed all the 3 stages of AKI. Difference was highly statistically significant (p= 0.001). Conclusion: Finding of the present study indicates that strict monitoring should be done in neonates on exclusive breastfeeding who exhibit warning signs and symptoms of both the conditions.