Kidneys are vital organs playing various functions important to sustain life. The key function of kidney is to remove waste material from body via filtration. If this ability of kidney is lost the resulting condition is called End Stage Renal Disease (ESRD). This is life threatening condition and requires either hemodialysis or Kidney transplant. The former need to be performed on regular basis, where as latter can be done once. However, transplant needs to be taken care by life time medications. As result of transplant and a treatment associated with it, there is an alternation in certain biochemical parameters. Oxidative stress is the state which has more burden of free radicals (oxidants) as compared with antioxidants. Antioxidants nullify the deleterious effects of free radicals. Oxidative stress has been found to play a crucial role in causation of many disease. The current study revealed how the markers of oxidative stress changes during transplant process. The study has been conducted on 31 kidney transplant recipients and 31 sex–age matched healthy controls. MDA levels were found to be significantly high in pre–transplant as compared to control but after transplant there was significant reduction noted. Whereas Total antioxidant capacity and other antioxidant enzymes have been found to increase gradually after transplantation. Our study also revealed mild effect of immunosuppressant regimen on oxidative stress.