IJSR International Journal of Scientific Research 2277 - 8179 Indian Society for Health and Advanced Research ijsr-9-4-24911 Original Research Paper EVALUATION OF SPOT URINE MICROALBUMIN AND GLYCATED HEMOGLOBIN LEVELS IN TYPE 2 DIABETIC SUBJECTS Arun Sharma Dr. April 2020 9 4 01 02 ABSTRACT

Background: Diabetes is a dysglycemic metabolic syndrome, widely prevalent in most of the developing and developed nations. As per International Diabetes Federation, there are about 382 million people worldwide living with diabetes, and India ranks among top three countries. Uncontrolled Diabetes leads to development of macrovascular and microvascular complications: the macrovascular complications include cerebrovascular accidents, coronary artery disease, peripheral vascular disease and the microvascular complications include nephropathy, retinopathy and neuropathy. Aim and Objectives: The objectives of our study include: 1) To estimate the spot urine microalbumin and glycated haemoglobin levels in subjects with type 2 diabetes and 2) To study the prevalence of normoalbuminuria, microalbuminuria and microalbuminuria depending on the duration of Diabetes. Materials and Methods: This cross–sectional study was conducted at Dept. of Medicine, World College of Medical Sciences and Research. A total of 100 non–diabetic subjects and 100 type 2 diabetic subjects were included in the study. Sample collection and analysis: Early morning mid–stream urine sample was collected and used for urine microalbumin and creatinine estimation. HbA1c was measured by HPLC method: Assay is based on Ion–Exchange HPLC. Results: A total of 100 non–diabetic subjects as controls and 100 type 2 diabetes subjects were studied. Out of the 100 controls 56 were males, 44 were females and among 100 type 2 diabetic subjects 58 were males and 42 were females. Urine spot albumin levels were evaluated in type 2 diabetic subjects and found that 52% subjects had urine albumin in normoalbuminuria range, 36% in microalbuminuria range and 12% had macroalbuminuria range. Further depending on the duration of diabetes microalbumin levels were assessed and found that 29% microalbuminuria was present with diabetes duration of 5–10 years. Conclusion: Diabetic Nephropathy is one of the leading causes of end stage renal disease. Early detection of diabetic nephropathy relies on tests for urinary excretion of albumin. Annual screening of microalbuminuria is recommended for all diabetic patients. Many studies have confirmed that an angiotensin–converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB) can retard the progression of albumin excretion.