BACKGROUND: Cervical cancer is the leading cause of morbidity and mortality among women worldwide. HPV with their oncogenic strains is virtually the primary cause for it. Cytology and HPV testing are known to be effective methods to detect cervical cancer and could contribute to the reduction of disease in developing countries. AIM: In developing countries like India, occurrence of HPV in cervical cancer and in asymptomatic patients was observed to be very high. The present study was therefore aimed to determine the prevalence of genital HPV infection in women with abnormal cytology and also to determine the presence of HR genotypes in these females. MATERIAL AND METHODS: Fifty females with abnormal cytology in Gynae OPD of the Institute constituted the study group. The samples were collected with the help of a device provided in the kit. Tests were performed using HybriBio HPV kit which is designed for the detection as well as determination of 21 specific HPV subtypes. RESULTS: Out of total fifty females, 16 (32%) were positive for HPV DNA and 34 (68%) were negative. HPV 16 genotype was the commonest, present in 8 (50%) cases followed by type 18 present in 6 (37.5%) and types 39 and 52 present in 1 (6.25%) case each. Significant association was seen between HPV positivity and young age group(p=0.033), high parity(p= 0.001), multiple sexual partners(p=0.035), presence of other STI’s(p= 0.018) and use of condom(p <0.001). No statistically significant difference was seen between HPV positivity and age at marriage, coitarche and coital frequency. CONCLUSION: Needless to say, health education, cervical screening, promotion of condom usage and prophylactic vaccination before sexual activity is the most effective approach in reducing the incidence of cervical cancer in resource crunched societies