This study aims at assessment of knowledge, attitude and practice of health care professionals regarding pharmacovigilance in a tertiary care hospital. Study was conducted among 133 health care professionals of a tertiary care hospital in Kerala. Data was gathered using questionnaire containing 20 questions. Based on score of knowledge 25(18.8%), 71(53.4%), 37(27.8%) were in excellent, good and poor categories respectively. 98(73.7%) participants agreed that ADR monitoring will benefit the patients and 93(69.9%) considered it as a professional obligation. 86(64.7%) knew to fill ADR form and 80(60.2%) had reported ADR before. 119(89.5%) participants agreed to report ADR hereafter and most important discouraging factors for reporting ADRs were lack of time and fear of legal issues. Awareness of health care professionals regarding pharmacovigilance has improved over past 3–4 years. Pharmacists are the major group of health care professionals properly sensitized.