Objectives: The aim of the present study was to evaluate the effect of diode laser therapy on periodontal pockets with regards to its bactericidal abilities and to assess the improvement in periodontal health. Materials and Methods:This split mouth study included 30 sites from 15 patients, randomly designated to receive one of the treatment procedures. Group–A subjects (control) received scaling and root planing followed by normal saline irrigation. Group–B subjects received scaling and root planing along with diode laser application. The patients were followed up for a period of 90 days. The clinical parameters evaluated include, bleeding on probing, clinical attachment level and probing pocket depth. The sub–gingival plaque samples were collected for microbiological evaluation. Results: The results showed that there was a significant reduction in bleeding on probing and probing pocket depth along with gain in attachment levels in the test group. The results of the test group also revealed a significant bacterial reduction. Conclusion: Within the limits of the study our results indicate that the diode laser can constitute an excellent adjunctive device to the conventional method of scaling and root planning by exhibiting the anti–bacterial property of laser light.