Context: Correction of periodontal bone loss using GTR is an accepted approach. But absorbable membrane lacks flexibility and has limited osteo–conductive and osteo–inductive capability. These aspects require further improvement to better utilize barrier materials with natural source and contents similar to bone, non–invasive attainability, bio–compatibility, low risk of rejection and infection. Aims: To evaluate proliferation and attachment of dental pulp stem cells on demineralized dentin to be used as semi–rigid barrier for GTR. Methods and Material: Dentin was harvested from caries free extracted teeth and were stored in 10% formalin. They were sliced in the occluso–apical direction in cuboid form to acquire a thin block(2x2x8mm) of medial dentin. Three specimens from each group– untreated dentin, acid treated dentin, and collagen membrane were included. MTT assay was done to detect cell attachment and proliferation of dental pulp cells on each specimen. Pulp stem cells were incubated on the each specimen for 3 days and observed under microscope. Results: Cell attachment and proliferation of dental pulp stem cells were observed on acid treated dentin. Conclusions: Hence, we conclude demineralized dentin is bio–compatible and can be used in future as novel barrier membrane for guided tissue regeneration.