Objective: To study the biochemical changes and myalgia following the administration of succinylcholine (Sch) with or without pretreatment with rocuronium in patients undergoing tympanoplasty. Method: The present prospective, double–blind, randomised clinical study was conducted after receiving clearance from the institutional ethical committee. Using a closed envelope technique, 80 American Society of Anaesthesiologists (ASA) grade I/II patients aged 20−40 years scheduled for tympanoplasty under general anaesthesia were randomly divided into two groups, Group R and Group C, with 40 patients in each group. Group R patients were administered Sch with rocuronium pretreatment, whereas Group C patients were administered Sch without rocuronium pretreatment. Venous samples were drawn for serum creatinine phosphokinase levels and serum potassium levels preoperatively, intraoperatively, and 24 h postoperatively between the two groups using the unpaired t–test. Sch–induced fasciculation and postoperative myalgia are known to be a well–recognised side effects of it’s administration. Results: The mean creatinine kinase (IU/L) level 24 h postoperatively was significantly more among subjects in Group C than among those in Group R (P = 0.001). The mean potassium level 24 h postoperatively was significantly more among subjects in Group C (5.03 ± 0.90) than among those in Group R (4.79 ± 1.05), (P = 0.045). Conclusion: Pretreatment with the nondepolarising muscle relaxant, rocuronium, prior to the administration of Sch significantly reduced the side effects of Sch