Introduction: The etiology and pathogenesis of acute pancreatitis are not completely clear. Acute pancreatitis (AP) is an inflammatory condition varying from a mild self–limiting to a severe systemic disease. Findings indicated that Myeloperoxidase (MPO) level increases in patients with AP and hence these indicators can be used as diagnostic factors to predict inflammation severity in AP. Aim: This study aims to validate the role of activation of MPO and Malondialdehyde (MDA) as a biomaker marker in patients with Pancreatitis in Indian subcontinent. Method: 50 Patients of Pancreatitis attending general surgery OPD and admitted to General Surgery department of SSKM Hospital ,Kolkata, West Bengal, India were taken. ELISA was done for MPO and MDA assay which are directly proportional to the color intensity of the test sample. Statistical Analysis was performed with help of Epi Info (TM) 3.5.3. EPI INFO is a trademark of the Centers for Disease Control and Prevention. Result: Level of MPO was decreased in acute pancreatitis compared to chronic pancreatitis and normal pancreatitis which was statistically significant. We also found that MDA was increased in chronic pancreatitis compared to acute pancreatitis and normal pancreatitis though it was not statistically significant. Conclusion: Plasma Malondialdehyde and Myeloperoxidase may be a useful additional marker of severity in the very early stages of acute pancreatitis.