AIM: The aim of this study is to evaluate stereomicroscopically the microleakage of root end filling materials Mineral Trioxide Aggregate (MTA) and Biodentine. METHODOLOGY: Twenty extracted human premolars were obturated with gutta percha using lateral compaction technique. Following this, teeth were resected apically & standardized root end cavities were prepared. The teeth were then randomly divided into 2 groups of 10 specimens each & were filled with Group – I: MTA, Group – II: Biodentine. They were immersed in 1% methylene blue dye for 72hrs. The teeth were then sectioned longitudinally & examined under stereomicroscope. The depth of dye penetration was measured in millimeters. Results: Microleakage was found to be significantly less in Biodentine. CONCLUSION: The results suggest that both materials presented microleakage. However Biodentine showing the least microleakage of all.