Context: Hypercalcemia has been reported upto 4.2% to 25% of patients who have malignancy related to head and neck cancer. In Health and Diseases, alkaline phosphatase refers to the activity of serum liver and bone alkaline phosphatases that has been widely applied in routine diagnosis. Aim– The aim of this study is to find an association of serum level of Akaline Phosphatse and Calcium in Osteolytic Lesions of Mandible and Maxilla. To compare and evaluate the level of Serum Calcium and Serum Alkaline Phosphatase in Osteolytic Lesions of Maxilla and Mandible. Settings and Design: A total of 20 participants (10 benign patients and 10 malignant lesions) were included in the study. Materials and Methods: Fasting blood ALP activity was evaluated using ALP assessment kit and biochemistry analyzer. Statistical Analysis Used: The data were analyzed by SPSS‑21 software (SPSS Statistics software).