OBJECTIVE: The aim of the study was to identify bacterial isolates & it’s susceptibility pattern in southern Rajasthan. Archived pus culture data obtained from MBGH, RNT Medical College Rajasthan Sept.2017 to Sept.2018. MATERIAL & METHOD: A total of 211 pus samples were included in the study, from patient attended MBGH at R.N.T. Medical College, Udaipur. Bacterial isolates obtained were identified using various phenotypic methods (Biochemical reactions & Antibiotic susceptibility test). Antibiotic susceptibility test was performed by Kirby–Bauer disc diffusion method on Mueller–Hinton Agar. RESULT: Out of total of 211 sample; Staphylococcus aureus was most commonly isolated (24.6%) followed by Escherichia coli (17%), Pseudomonas sp. (10.8%), Methicillin sensitive staphylococcus aureus (9%), Methicillin resistant staphylococcus aureus (6%), Klebsiella (6%) among others. Among Gram positive cocci Amikacin was found to be highly sensitive and for Gram negative bacilli Meropenem was found to be highly sensitive. CONCLUSION: There was a high resistant rate toward different antibiotic & their study give a good guide to the clinician to propose an empirical treatment for the patients. Meropenem should be kept in reserve for resistant cases.