During Pregnancy genital tract colonisation is common. Those harbouring pathogenic organisms are asymptomatic prior to labour and may develop infection or sepsis during Intrapartum and postpartum. Neonatal sepsis is also acquired during the process of labour and delivery. To investigate this 2 groups of women (85 in each group) were screened for genital tract colonisation prior to induction of labour. Group1 consisted of women with risk factors for infection like diabetes and group 2 without risk factors for infection. Cervical, Vaginal, Rectal swabs were taken prior to induction of labour and subjected to bacterial culture on blood agar and selective media for Group B Streptococci. Cervical and Vaginal cultures were repeated during labour and immediate puerperal period. Puerperal and Neonatal sepsis were diagnosed as per clinical criteria. Infection was diagnosed on positive culture reports. Both the groups were compared for incidence of bacterial infection, bacterial profile and Maternal and Neonatal infection and Sepsis. Chi square test was done to compare proportions. The correlation between continuous variables was done using Pearson’s correlation coefficient. All analyses were done using 2 tailed hypotheses and p<0.05 was considered significant. Bacterial genital tract colonisation was present in 40.5% and it was not different in women with risk factors and without risk factors. But significantly more number of colonised women with risk factors developed infection during Intrapartum and postpartum period. Non– colonised women also developed infection but this was not significant when compared to Colonised women. The most common organism was Escherichia Coli and Polymicrobial. Puerperal sepsis and Neonatal sepsis occurred in 15% and 8% respectively. It is desirable to screen pregnant women with risk factors for infection during Intrapartum or postpartum period and or adopt prophylactic antibiotic therapy to prevent sepsis. Group B Streptococcus (GBS) colonisation or sepsis is very rare in this cohort of South Indian Pregnant women