Aim: To determine serum metabolites viz. calcium and electrolytes levels in newborns with birth asphyxia. Materials and methods: This study done at IQ City Medical College and NH multispeciality Hospital comprised of 58 neonates with birth asphyxia and 38 in control group. The serum calcium and electrolytes were recorded. The two groups were compared using unpaired student’s t test. Result: The mean serum calcium, sodium and potassium levels were 7.96±0.66 mg/dl, 136.76±5.6 meq/L and 5.33±0.89 meq/L in cases. In control group these were 9.5±0.64 mg/dl, 134.97±5.44 meq/L and 5.27±0.99 meq/L respectively. In birth asphyxia cases serum calcium was significantly low (p < 0.005). The serum sodium and potassium levels in both the groups were parallel. Conclusion:Early diagnosis and proper treatment of metabolic changes commonly occurring in neonates with birth asphyxia may improve their life and also decrease the mortality rate.