Introduction:

Resistance to antimicrobial agents (AMR) has resulted in increased morbidity and mortality from treatment failures and increased health care costs. Although defining the precise public health risk and estimating the increased costs is not a simple undertaking, there is little doubt that emerging antibiotic resistance is a serious global problem. The present write–up enumerates various methods for detecting the

resistance of common bacteria to various antimicrobial agents as available today. Antibiotic therapy is usually given to protect from infection against various organisms, however, routine use of empirical treatment has resulted in widespread antibiotic resistance and development of antibiotic resistant genes.

Objective: To identify the antimicrobial sensitivity and resistance pattern of bacterial isolates among clinical samples from the ICU patients.

 Materials & Methods: All the clinical specimens submitted to microbiology laboratory were processed as per standard microbiological procedures and antibiotic sensitivity tests were performed on the isolates as per guidelines of clinical and laboratory standards institute (CLSI).

Result:

A total of 250 organisms were isolated from all clinical specimens, out of which 113 were Gram positive and 137 were Gram negative bacteria. Amikacin showed high degree of sensitivity among Gram positive bacteria as well as in Gram negative bacteria. Gram positive bacteria showed highest degree of resistance to penicillin and erythromycin, whereas, Gram negative bacteria showed highest degree of resistance to Cefuroxime.

Conclusion:

High frequency of resistance against commonly used antibiotics such as penicillin and cefazolin as shown in the

present study indicates a serious problem in the treatment of infections by gram positive and negative organisms. Therefore

continuous surveillance is needed and treatment based on antibiogram report is essential.