Cytomegalovirus (CMV) infection in pregnancy is too common occurring primarily as well as recurrently due to reactivation of latent infection. The primary CMV infection during pregnancy is associated with numerous complications . Infection is routinely diagnosed by microscopy( pathognomonic owl’s eye intranuclear inclusions ) which is further confirmed by other methods like immunofluorescence assay , ELISA assay , CFT , PCR or in situ DNA probe hybridization assays which is used to detect viral DNA in biopsies, blood, bronchoalveolar lavages, and urine samples . Aims & objective : Screening of urine sample of pregnant women for CMV infection in a tertiary care hospital . Results : A total of 104 urine samples of pregnant women coming for ANC were screened for CMV by microscopy between May 2017 and August 2017 . There were 12 samples (11.54%) which were positive by direct microscopy . Of these pregnant females , 21 cases ( 20.19 %) were positive by serological test for CMV infection , which included the cases which were positive by microscopy . Conclusion: There should be a mandatory screening of all females in child bearing age group for primary CMV infections .