Comparison of high risk human papilloma virus DNA testing and pap smear cytology for screening of cervical cancer in high risk group of patients

Dr. Kumkum Gupta

Volume : VIII, Issue : VII, July - 2019

Comparison of high risk human papilloma virus DNA testing and pap smear cytology for screening of cervical cancer in high risk group of patients

Dr. Kumkum Gupta

Abstract :

Objective: To compare high risk HPV DNA testing and conventional pap smear cytology for screening of cervical cancer in high risk group of patients. Methods: A hospital based prospective, comparative and observational study conducted in department of Obstetrics and Gynecology, Government medical college, Kota on 150 eligible cases who were sexually active between age group 30-65 years. All eligible women underwent Pap‘s smear testing and HPV DNA testing from ecto-cervix and endo-cervix for screening of carcinoma cervix. Result: Out of all 150 cases, 11 cases were positive for HPV DNA testing. Pap‘s smear was positive in 29 cases. ASCUS were 14%, LSIL were 2.67%, HSIL were 2% and SCC 0.66%.all LSIL, HSIL cases were HPV positive and all 21 ASCUS positive cases were HPV DNA test negative. Colposcopy was done in 11 HPV DNA positive cases, we found that 3 were having grade 1 colposcopy and 4 were having grade 2 colposcopy and 4 were having grade 3 colposcopy and all 4 slides were cytological positive (HSIL).colposcopy was done for Pap‘s smear positive cases (ASCUS and higher).Out of 4 LSIL cases, 2 were grade 1 and 2 were grade 2 and all HSIL were showing grade 3 colposcopy. In HPV positive cases, colposcopy was significant in 72.72% cases and in Pap‘s smear positive cases colposcopy was significant in 28.57% cases, we found 3 HPV positive and cytology negative cases. Conclusion: In comparison to conventional Pap‘s smear, HPV DNA test is more superior for detection of missed cervical abnormalities and women diagnosed as HR -HPV DNA positive needs close surveillance and treatment as required.