DESTAINING EFFECT OF ALKALINE PROTEASE PRODUCED BY PD4 STRAIN OF BACILLUS SUBTILIS

N.Vanitha; S.Rajan; AG.Murugesan

Volume : VII, Issue : IX, September - 2018

DESTAINING EFFECT OF ALKALINE PROTEASE PRODUCED BY PD4 STRAIN OF BACILLUS SUBTILIS

N. Vanitha, S. Rajan, Ag. Murugesan

Abstract :

The demand for alkaline proteases in industries is increasing worldwide. The present research describes the production of alkaline protease in a lab scale fermentor from PD4 strain of Bacillus subtilis isolated from effluent. The production of alkaline protease was enhanced by optimizationof cultural condition. Of all proteases,alkaline proteases are of great importance in detergent industry due to their high thermal stability and pH stability.Ability of the alkaline protease to destain different stains on the cotton faic samples wereevaluated using four different stained clothes. Destaining was done using 10 ml of crude preparations. Crude alkaline protease showed good destaining ability when compared to that of other three test faics treated with distilled water, distilled water with detergent, enzyme extract with detergent respectively and it was revealed that the purified enzyme can be used as an additive in detergent industry.