DIFFERENCE BETWEEN AMPLICOR AND LINNEAR ARRAY TESTS FOR DETECTING HPV- INDUCED CERVICAL ABNORMALITIES

Krivak-Bolanca I.; Findri Gustek S.; Orescanin V.

Volume : V, Issue : III, March - 2016

DIFFERENCE BETWEEN AMPLICOR AND LINNEAR ARRAY TESTS FOR DETECTING HPV- INDUCED CERVICAL ABNORMALITIES

Krivak Bolanca I. , Findri Gustek S. , Orescanin V.

Abstract :

The aim of the study was to morphologically analyse cervical samples prepared with liquid-based cytology using transport medium residual samples collected for the detection of hr-HPV, and to genotype the samples by the reverse hyidization strip assay in each specimen. For detection of viral infection and detailed genotyping the Amplicor assay and Linnear Array methods were used. Cytological diagnoses were made according to the Bethesda classification. Cell abnormalities were observed in HPV positive samples, but also in samples that tested HPV negative. Detection of HPV infection showed infection with one viral type but in most cases infection was caused by more viral types. Between those two tests, difference was noticed, reflecting on the frequency and on the appearance of existing low and high grade cervical lesion. The sensitivity of these tests has been observed to be somewhat different, though not statistically significantly.