Evaluation of PCR as diagnostic tool in Pediatrics Pulmonary tuberculosis

Padmanabhan R; Arunachalam V; Heber Anandan; Gandhimathi S

Volume : V, Issue : VIII, August - 2016

Evaluation of PCR as diagnostic tool in Pediatrics Pulmonary tuberculosis

Padmanabhan R, Arunachalam V, Heber Anandan, Gandhimathi S

Abstract :

Introduction: Tuberculosis even today, remains major health problem among children in the world especially in India. Amplification of M. tuberculosis specific DNA sequences (IS 6110) in clinical samples is the most sensitive and rapid method of detection available 56677. Aim: Evaluation of PCR as a diagnostic tool in Pulmonary Tuberculosis in children Methods: Observational diagnostic study was done in children diagnosed with pulmonary tuberculosis. These specimens were subjected for microbiological studies and PCR studies. M. tuberculosis culture was done using LJ medium. Results: 32 children were included in the study with pulmonary tuberculosis. Microbiological method has shown negative to all clinically confirmed cases. PCR shown 25 cases positive among 32 cases. Conclusion: Polymerase chain reaction in diagnosing pediatric pulmonary tuberculosis cases is superior to conventional microbiological method