Volume : VI, Issue : VII, July - 2017

Microbial Diversity analysis of Dal lake, India using 16S rRNA Gene Based Culturing Approach.

Khan J. A

Abstract :

 Dal lake ecosystem (Lat. 340-6’ N, 740-45’E, alt.1583) situated in the heart of Srinagar city the summer capital of Jammu and Kashmir state is under tremendous anthropogenic pressure for the last there decades.More than 50,000 people are living inside this hamlet, besides some people residing inside houseboats. People use this lake  for their own purposes as this lake acts as livelihood for many families since centuries. Little work had been carried on Dal lake water and sediment samples to show the type of  Microbial Diversity existing at present. By our work we tried to show whether anthropogenic activity is responsible for producing harmful bacteria. The traditional method of Microbial diversity analysis, culture dependent identification of species through morphological and biochemical test has not given the oad range of organism present in Dal Lake.  Till date, only Escherichia coli, Salmonella typhimurium and Penicillium sp. were only identified. We have utilized 16S rRNA approach  first time to study Microbial diversity in detail by taking water and sediment samples and had grown them on different media like Nutrient agar, Blood agar, and Eosine Methyline blue agar.  As studies of 16S rRNA provides a microbial taxa present in a given sample because it is an excellent phylogenetic marker. Bacterial 16S rRNA sequences show the distribution of microorganisms predominantly in Bacillus, Acinetobacter and Pseudomonas species. As by our findings sediment samples show a variety of bacteria, whereas water samples showed presence of very few types of bacteria. The other bacteria recovered from the sediment samples include bacteria belonging to Sporosarcina, Streptomyces, Arthrobacter, Rhodococcus, Enterobacter, Hydrogenophaga, Rheinheimera, and Gammaproteobacterium species. The other isolate recovered from the water samples belong to the species Comamonas, Micrococcus, Rheinheimera, Acidovorax, Sporosarcina, Paenisporosarcina, Rhodococcus, Bosea, Arthrobacter. Based on literature survey, we can utilise the services of these bacteria for various  purposes as these  microorganisms can ing bioremediation activity in Dal lake such as degradation of crude oil, aromatic and alicyclic compounds, polynuclear aromatic hydrocarbons, mineral oils, synthetic polymers, styrene, heavy metals. 

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Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

Khan J.A, Microbial Diversity analysis of Dal lake, India using 16S rRNA Gene Based Culturing Approach., GLOBAL JOURNAL FOR RESEARCH ANALYSIS : VOLUME-6 | ISSUE‾7 | JULY -2017

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