A study of Neutrophil CD64 expression by Flowcytometry in cases of sepsis- A Hospital based study.

Dr.Bidit K. Gogoi; Dr. Rasadhar Deka; Dr. Nitu Mani Khakhlari; Dr. Bibhash Gogoi

Volume : VIII, Issue : V, May - 2019

A study of Neutrophil CD64 expression by Flowcytometry in cases of sepsis- A Hospital based study.

Dr. Bidit K. Gogoi, Dr. Rasadhar Deka, Dr. Nitu Mani Khakhlari, Dr. Bibhash Gogoi

Abstract :

Diagnostic indicators of the systemic acute inflammation response to infection or sepsis have shown few innovations in the past few decades. The usual laboratory tests employed to diagnose systemic infections such as leukocyte count, presence of immature leukocyte forms (bands), C reactive protein (CRP), and erythrocyte sedimentation rate (ESR), have poor sensitivity and specificity. Microbiological cultures are time consuming, often negative in those who are receiving antibiotics, and depend on various technical factors, such as the timing and technique of specimen collection and the transport time to the laboratory. CD64 is the Fc receptor (Fcg receptor I Fcg RI) on Neutrophils which is constitutively expressed on macrophages, monocytes, and eosinophils. It is up regulated on neutrophils as a physiological response to microbial wall components and some cytokines. Up regulation of CD64 occurs within four to six hours after stimulation with interferon γ or granulocyte colony stimulating factor 4 and other activators, such as lipopolysaccharides Aims: Our study aimed to demonstrate CD64 expression in cases of Sepsis. Settings and Design: This is a Prospective Hospital based study in a Tertiary Hospital. Methods and Material: We carried out the study in 100 suspected cases of sepsis with an equal number of age and sex matched control using fluorochrome APC H7 for CD45, PE for CD64 and FITC for CD14 by multiparametric flowcytometry. Statistical analysis used: P Value was calculated statistically by using CHISQUARE TEST. For Statistical convenience, MFI 80 was taken as cut off value. Results: Out of 100 cases we found True Positive of 89% and False Negative of 11%. Out of 100 controls we found False Positive of 10% and True Negative of 90% which showed accuracy of 93.72%. Conclusions: Our study demonstrates that neutrophil CD64 expression by Flowcytometry can be a diagnostic tool in cases of sepsis. The neutrophilic CD64 (nCD 64) expression was measured by estimating the median flurochrome intensity (MFI).