Bacteriological profile and Antibiogram of isolates from Pus samples in a Tertiary care center.

Dr. Neelam Chauhan

Volume : VIII, Issue : V, May - 2019

Bacteriological profile and Antibiogram of isolates from Pus samples in a Tertiary care center.

Dr. Neelam Chauhan

Abstract :

Background: Pus is one of the major samples received in the Microbiology laboratory for culture and sensitivity. Objective: Study of bacterial isolates from the pus samples and their sensitivity pattern so that it will help the clinicians to treat patients and help the hospital to have its antibiotic policy. Method: This study was conducted in the Department of Microbiology, Pacific Institute of Medical Science(PIMS),Udaipur (Rajasthan) for a period of 3months.Pus samples from different department of hospital were collected using aseptic technique and were processed in the laboratory immediately using standard microbiological procedures .Identification of bacteria was carried out with motility testing, Gram staining and biochemical reactions. The antibiotic sensitivity testing of all isolates was performed by Kirby Bauer’s disc diffusion method on Muller Hinton agar and interpreted as per CLSI guidelines. Results : Analysis of 100 pus samples showed 41.66% culture positivity. Among the 100 culture positive cases 62 (62%) were male and 38 (38%) were female with Surgical wards (34%) being the major contributor. Staphylococcus aureus was the major bacterial isolates (38%) with Pseudomonas aerugionsa being the second predominant one (22%).The prevalence of MRSA was 36.84% .Gram positive cocci were 100% susceptible to Linezolid and vancomycin followed by Doxycycline where as Gram negative bacilli were most susceptible to Colistin ,Imipenem and Amikacin, Gentamicin. Conclusion: Changing antimicrobial resistance pose challenge in treating pyogenic infections .Appropriate and judicious selection of antibiotic would limit the emerging drug resistant strains in the future to treat these clinical conditions successfully.