Biochemical characterization and 16S rRNA sequencing of probiotic strain isolated from cow milk

Balasubramanian.R; Narayanan.K.R; Sornam.R

Volume : VII, Issue : XII, December - 2018

Biochemical characterization and 16S rRNA sequencing of probiotic strain isolated from cow milk

Balasubramanian. R, Narayanan. K. R, Sornam. R

Abstract :

16S rRNA gene sequencing approach to identify a novel species of Probiotic Leuconostoc pseudomesenteroides . The cow milk sample was collected from alwarkurichi, Tirunelveli District, Tamil nadu, India. Subsequently the sample was serially diluted and the aliquots were incubated for a suitable time period following which the suspected on colony was subjected to biochemical characterization, antibiotic sensitivity assay and 16S rRNA sequencing. The sequence aligned against other species were concluded to be a novel, Probiotic Leuconostoc pseudomesenteroides bacteria, further which were named Leuconostoc pseudomesenteroides strain RBSS10. After the sequence characterization, the isolate was deposited in GenBank Database, maintained by the National Centre for Biotechnology Information NCBI. The sequence can also be retrieve from EMBL and DDBJ repositories with accession number MK290325.