BIODEGRADATION ASSESSMENT OF OIL CONTAMINATED SOIL AT THANJAVUR DISTRICT

Mohan Doss. R; Rajeswari. B; C. Sivasubramanian

Volume : VIII, Issue : IV, April - 2019

BIODEGRADATION ASSESSMENT OF OIL CONTAMINATED SOIL AT THANJAVUR DISTRICT

Mohan Doss. R, Rajeswari. B, C. Sivasubramanian

Abstract :

Soil samples were collected from different sites of Thanjavur city. Biosurfactants possess both hydrophobic and hydrophilic moieties, they are able to reduce surface tensions and interfacial tension between two fluids at the surface and interfere respectively. We isolated soil bacteria and screened them for biosurfactant activity. The extracts were then separated by thin layer chromatography, oil spreading techniques and Restriction fragment length polymorphism. In oil spread techniques, 20 µl oil was fully degraded and yellow spot was observed by TLC in all the samples. The restriction fragment length shows 2 fragment (petrol bunk and oil mill shop), 4 (mechanical work shop) and 3 (Engine oil mill). The compounds will be further assessed for their activities in oil degradation.