Chromatographic separation of phytoconstituents from Sesuvium portulacastrum.

Snehal B. Gagare; Pratima S Jadhav.

Volume : VII, Issue : IV, April - 2018

Chromatographic separation of phytoconstituents from Sesuvium portulacastrum.

Snehal B. Gagare, Pratima S Jadhav.

Abstract :

The bioactive components were separated fromSesuvium portulacastrum(Aizoaceae family) a halophyte known to beused as a traditional medicine.The present study focuses on Chromatographic characterisation of the halophyte using TLC ,Column chromatography and HPLC. Evaluation of solvent system for chromatographic separation revealed that Ethylacetate:aceticacid:water (5:1:1) is the most appropriate solvent system, for separation of bioactives. From the crude extract of halophyte, 20 fractions eluted by column chromatography and were confirmed by TLC to have specific Rf value . The 3 different eluted fractions, were further characterized by HPLC which illustrateshighest peak values at 2.885 , 3.027 ,2.093.