COMPARATIVE STUDY OF HOLOTRANSCOBALAMIN AND TOTAL VITAMIN B12 AS INDICATOR OF VITAMIN B12 STATUS

Dr Radhika Chowdhary; Dr Priyanka Bhatkulkar

Volume : VII, Issue : VIII, August - 2018

COMPARATIVE STUDY OF HOLOTRANSCOBALAMIN AND TOTAL VITAMIN B12 AS INDICATOR OF VITAMIN B12 STATUS

Dr Radhika Chowdhary, Dr Priyanka Bhatkulkar

Abstract :

Vitamin B12 deficiency is common among the population and early detection is clinically important. HoloTC has been postulated to be the earliest marker of vitamin B12 deficiency. This study aims to evaluate the usefulness of holoTC compared to Total Vitamin B 12 in diagnosing B12 deficiency in patients investigated for Vitamin B12 deficiency. We randomly selected 65 patients visiting the outpatient department of KIMS Hospital Secunderabad and who had been referred to the Clinical Biochemistry Laboratory, for the assessment of vitamin B12 status, during September 2017. serum total vitamin B12 level was determined by the Electrochemiluminescenceimmunoassay (ECLIA) on cobas 6000 immunoassay analyzer.andholoTC (active vitamin B12) level was determined by micro particle enzyme immunoassay on Beckman analyzer using AxSYM Abbott kit. Comparison of the data was performed to test the statistical relationship as reflected by the mean, median, standard deviation and correlation coefficient. The mean (SD) for serum holoTC and total vitamin B12 were 24.48(10.32) pmol/L and 131.46(41.76) pmol/L respectively. There was weak correlation between holoTC and total vitamin B12 (r= 0.443, P< 0.001) and the regression equation was expressed as; y = a + bx (i.e: holoTC = 11.08 + 0.1Total vitamin B12). holoTC and total vitamin B12, alone and in combination, have almost equal diagnostic efficiency in diagnosing vitamin B12 deficiency for the majority of patients. Even if there is weak correlation between the two majority of patients had agreement over either normal or low values of vitamin B12 as well as holoTC.< clear="all" style="page–eak–before:always;mso–eak–type:section–eak" />