Efficacy of Dilute Povidone-Iodine Preparations Against Multi-Drug Resistant Biofilms of Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans

Tessema B; Wycherly B; Arumugam S; Capriotti K; Pelletier J; Barone S; Capriotti J

Volume : VII, Issue : I, January - 2018

Efficacy of Dilute Povidone-Iodine Preparations Against Multi-Drug Resistant Biofilms of Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans

Tessema B, Wycherly B, Arumugam S, Capriotti K, Pelletier J, Barone S, Capriotti J

Abstract :

Objective: To study the in vitro effect of novel low–dose povidone–iodine formulations against established biofilms of multi–drug resistant Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans. Methods: Biofilms of multi–drug resistant Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans were developed on solid surfaces using the Calgary Biofilm Device (CBD) plate. Minimum biofilm eradication concentration (MBEC) was then determined for each test drug and for control samples of known antibiotics, ciprofloxacin and itraconazole. Results: The low–dose povidone–iodine formulations completely eliminated all biofilms of bacterial and fungal species in the test systems. Ciprofloxacin was able to eradicate one bacterial biofilm only at concentrations greater than 0.25 ug/mL. Conclusions: These novel dilute PVP–I formulations are effective anti–biofilm agents in vitro. Further evaluation in living models is warranted.