Histopathological Changes in Developing Mice Kidney Treated with Lamotrigine on Early and Late Phase of Gestation

Shweta Singh; S.K. Pandey

Volume : VII, Issue : X, October - 2018

Histopathological Changes in Developing Mice Kidney Treated with Lamotrigine on Early and Late Phase of Gestation

Shweta Singh, S. K. Pandey

Abstract :

INTRODUCTION–Lamotrigine (LTG) is an anti–epileptic drug (AED) and also used as a neuromodulator, in mood disorders.Metabolism is primarily achieved by competitive glucuronic acid conjugation. The principal product is an inactive lamotrigine–2–N–glucuronide conjugate, found predominantly in urine and to a lesser extent in feces. Recently, malformations have been reported in human foetuses, whose mothers were treated with LTG. However, it is not been possible to establish a recognizable pattern of malformations in the kidneys of human foetuses treated with LTG, therefore the lower animal has been used as experimental model for the present study.

OBJECTIVE– The objective of this study is to find out the microscopic changes in the kidney of the growing emyo of mice treated with LTG in both early and late phase of gestation.

MATERIAL AND METHODS –The pregnant mice were divided into two experimental group’s i.e. early and late, each with two subgroups i.e. control group (treated with intra peritoneal injection of normal saline on Day 4 and Day 9 of gestation) and treated group (treated with intraperitoneal injection of LTG ,150mg/kg body weight on 4^th day and 9^th Day of gestation. Fetuses were collected on day 18^th of gestation and after fixation the kidneys were dissected out and processed for histological examination.

RESULT – The results of this study indicate that LTG administered intra peritoneally at high doses causes shrinkage of cortex and medulla and degenerated glomeruli, shrinkage of glomeruli and appearance of edematous spaces between the ducts.

CONCLUSION– In the present study histopathological changes were seen to be caused by LTG in the kidney of developing mice.