Identification and gene detection of common Salmonella serotypes in meat and poultry products from Egyptian markets

Nashwa Fawzy Abd El Moez Azzam

Volume : VIII, Issue : II, February - 2019

Identification and gene detection of common Salmonella serotypes in meat and poultry products from Egyptian markets

Nashwa Fawzy Abd El Moez Azzam

Abstract :

This investigation aims to identify and screen Salmonella serotypes isolated from one hundred meat and poultry products collected from local Egyptian market in 2018–2019. Morphological and biochemical identification were applied. Salmonella strains were highly susceptible to Ciprofloxacin (77.7% and 81.8%), kanamycin (72.2% and 63.6%), Ampicillin (55.5% and 45.4%) and Norfloxacin (38.3% and 63.6%) for poultry and meat products respectively. Then PCR was carried out to detect invA gene in isolated Salmonella. It was found that 36% (18/50) and 22% (11/50) of meat and poultry samples were positive for Salmonella isolation. Based on invA sequence alignments data, Salmonella serotype which isolated from meat products were identified as Salmonella newland (12.5%), Salmonella enteritidis (77.7%) and Salmonella hessarek (9.8%). On the other hand, Salmonella serotypes which isolated from poultry products were identified as Salmonella typhimurium (22.8%), Salmonella enteritidis (65.4%) and Salmonella Kentucky (11.8 %). Our obtaining results add more highlights for hygienic measurements to decrease meat and poultry products contamination with pathogenic Salmonella