Implementation of Non-Microscopic Method for Malaria Diagnosis

Dr. Nazir Abdul Wasim; Dr. Arijit Majumdar; Dr. Soumali Biswas; Dr. Swagata Bhattacharyya

Volume : VIII, Issue : VI, June - 2019

Implementation of Non-Microscopic Method for Malaria Diagnosis

Dr. Nazir Abdul Wasim, Dr. Arijit Majumdar, Dr. Soumali Biswas, Dr. Swagata Bhattacharyya

Abstract :

Rapid diagnostic tests for malaria are now a commonly used procedure for malaria diagnosis. Despite some problems related to sensitivity and applicability, malaria rapid diagnostic tests (RDTs), are currently considered the best option to overcome well trained experts in peripheral blood smear diagnosis. The present study was done to compare the non–microscopic methods like centrifuged buffy coat smear( CBC) and Antigen kit test(RDT) with the conventional peripheral blood smear for the rapid diagnosis of malaria which is essential to prevent the morbidity and mortality especially in endemic areas. A total of 100 samples were collected from patients presenting with classic symptoms of malaria. For traditional microscopy; thick and thin smears were prepared and stained with Leishman‘s –Giemsa stain. Antigen detection were done using commercially available kits. Malaria was diagnosed in 60, 62 and 65 patient by thick smear, CBC and antigen card test respectively. In antigen card test the sensitivity 91.6%, specificity 75%, Positive predictive value (PPV) 84.62% and Negative predictive value (NPV) 85.71% were observed. Although the antigen card test is superior than thin smear and CBC. Antigen card test has its advantages in terms of speed, sensitivity and specificity especially in an endemic area